escherichia coli Raw sequence reads

In this study, we aimed to engineer and evoluted Escherichia coli for improving methanol assimilation. Strains were firstly cultivated in Hi-Def Azure (HDA) medium, a semi-minimal medium containing amino acids. The percent of HDA was gradually reduced and replaced by M9 minimal medium during the evolution. After 12 passages, the culture can grow on methanol with 5% HDA while strain X5 cannot grow on methanol with less than 15% HDA.