Filamentous growth of elm1Δ fus3Δ cells.

All of indicated strains (MY8092, 12158, 12886 and 12948) were BY4741-derivative haploids and grown to exponential phase in liquid YPD at 30°C unless stated otherwise. (A) Arrows indicate the buds that emerge from the pole opposite previous division site. (B) elm1Δ fus3Δ (MY12948) harboring empty vector (MR1865) or pCEN-FUS3 (MR5048) were grown in liquid synthetic complete (SC) medium containing 2% glucose. (C) Cells were streaked for single cells on YPD plate at 30°C for 7 days, and representatives in a zone of low colony density were photographed. (D) Indicated cells (MY8092, 12158, 12886 and 12948) were streaked on YPD solid medium and incubated at 30°C. (E) The ratio of long axis (x) to short axis length (y) was expressed in a histogram. For example, 1.1 and 1.3 at the x-axis indicate the range from 1.00 to 1.19 and 1.20 to 1.39, respectively. (F) Flocculation assay was performed as described in Materials and methods. Data are representative of three independent experiments and expressed relative to WT. (G) Cells were spotted on YPD plate and grown at 30°C for 6 days. Haploid Σ1278b (MY13465) was used as a positive control of invasive growth. (A and B) Bar, 10 μm.

所指示的菌株（MY8092、12158、12886及12948）均为BY4741衍生的单倍体，除非另有说明，均在30°C的液体YPD培养基中培养至指数生长期。（A）箭头指示自上次分裂位点相对的极端出现的芽。（B）elm1Δ fus3Δ（MY12948）携带空载体（MR1865）或pCEN-FUS3（MR5048），在含有2%葡萄糖的液体合成完整（SC）培养基中培养。（C）细胞在30°C的YPD平板上划线，培养7天以获得单细胞，并在低菌落密度区域内选取代表性样本进行拍照。（D）指定的细胞（MY8092、12158、12886及12948）在YPD固体培养基上划线，并在30°C下进行培养。（E）长轴（x）与短轴长度（y）的比率以直方图形式表达。例如，x轴上的1.1和1.3分别表示从1.00至1.19以及从1.20至1.39的区间。（F）絮凝试验按照材料和方法中所述进行。数据代表三次独立实验的结果，并与野生型（WT）进行比较表达。（G）细胞在YPD平板上点种，并在30°C下培养6天。单倍体Σ1278b（MY13465）用作侵袭性生长的阳性对照。（A和B）条形表示10 μm。