Toxicity of oxyPAH to developing red drum (Scianeops ocellatus)

Data includes measures of mitochondrial function, cardiac edema, and corresponding PAH chemistry in control and oil-exposed fish. This study used naturally weathered slick oil from the surface (OFS) collected from surface skimming operations in the Gulf of Mexico following the Deepwater Horizon oil spill. Larvae were exposed to a diluted high energy water accommodated fraction (HEWAF) of oil and artificial seawater. Artificial seawater (27.6-28.0 ppt salinity, 25.3-26.7 °C, 7.15-7.16 mg/L DO, pH 8.22-8.30) was used in all control solutions and in preparation of treatment dilutions. Fifty PAH analytes were quantified by ALS Environmental using gas chromatography-mass spectroscopy in single-ion monitoring mode (GC/MS-SIM) according to US EPA method 8270D. The experiment was performed using a full factorial design with oil, UV, and exposure time as the effect variables. At each time point (0, 24, 48 h exposure), 3 replicates of 20 larvae were collected for transcriptomic analysis and 2 replicates of 10 larvae were collected for histological analysis. A terminal deoxynucleotidyl transferase (TdT) dUTP nick-end labeling (TUNEL) assay was used to stain apoptotic DNA. This assay was performed using the ApopTag Red in situ Apoptosis Detection Kit (Millipore) according to manufacturer’s instructions. Two sections for each larvae (n=6-10 per treatment) were used for the TUNEL assay. Images were taken on an EVOS FL Color Imaging System (Life Technologies) and were analyzed using ImageJ software (version 1.47).本数据集涵盖对照组与石油暴露组鱼类的线粒体功能、心脏水肿相关检测指标，以及对应的多环芳烃（PAH）化学分析数据。本研究使用的是深水地平线（Deepwater Horizon）漏油事件后，从墨西哥湾海面撇油作业中收集的天然风化海面浮油（OFS）。实验将鱼类幼体暴露于经稀释的石油高能量水可溶组分（HEWAF）与人工海水混合体系中。所有对照组溶液及处理组稀释液的配制均采用人工海水，其水质参数为：盐度27.6~28.0 ppt，温度25.3~26.7 ℃，溶解氧（DO）7.15~7.16 mg/L，pH值8.22~8.30。ALS环境实验室采用气相色谱-质谱联用单离子监测模式（GC/MS-SIM），依据美国环境保护署（US EPA）方法8270D，对50种多环芳烃待测物进行定量分析。本实验采用全因子实验设计，以石油暴露、紫外照射与暴露时长作为效应变量。在各采样时间点（暴露0、24、48 h），分别收集3组各20尾幼体用于转录组学分析，以及2组各10尾幼体用于组织学分析。采用末端脱氧核苷酸转移酶（TdT）介导的dUTP缺口末端标记（TUNEL）技术对凋亡DNA进行染色。本实验使用默克（Millipore）旗下的ApopTag Red原位细胞凋亡检测试剂盒，严格按照试剂盒说明书进行操作。每条幼体取两张组织切片（每个处理组n=6~10）用于TUNEL实验。成像采用EVOS FL彩色成像系统（Life Technologies），并使用ImageJ软件（版本1.47）进行图像分析。