Progressive Vulnerability of Cortical Synapses in α-Synucleinopathy: Dataset

Tabular data for the following experiments: pSyn pathology progressively increases_SS: Lewy pathology progression at 1, 2, and 3 months post–striatal PFF injection was quantified. pSyn pathology negatively impacts excitatory transmission_JG: Electrophysiology data from cortical slices were analyzed to assess how pSyn pathology affects synaptic transmission. Electron Microscopy analysis of excitatory synapses_JR_DR: Ultrastructural measures were quantified, including synaptic vesicle area, intervesicular distance, and synaptic cleft distance. SEQUIN for VGLUT1, VGLUT2 and VGAT positive synaspes_SS: Synaptic loci frequency distributions and synapse densities for VGLUT1- and VGLUT2-positive excitatory synaptic loci and VGAT-positive inhibitory synaptic loci were quantified. VGLUT1 and VGLUT2 colocalization with endogenous α-synuclein_SS: Colocalization of VGLUT1 and VGLUT2 with endogenous α-synuclein was quantified in the cortex. Analysis of synaptic localization of pSYN, synaptic density, and association of synaptic pSYN to pSYN positive neurites_AS: Synaptic localization of pSYN, synaptic density, and puncta size changes (relative to Lewy neurites), as well as the association of synaptic pSYN with pSYN-positive neurites, were quantified.