Effect of Rps5 heterozygous deletion on embryonic stem cells transcriptome. Mus musculus

Using wild-type and Rps5 heterozygous embryonic stem cells, we isolated RNA from polyribosomal fractions in order to get insights into transcriptional and translational defects of such deletion. Overall design: Input, monosomes and polysomes extracted RNA samples from wild-type and Rps5 heterozygous clones (undifferentiated and differentiated, total number of samples = 12), were subjected to sequencing.