RNA sequencing of P19CL6 cells expressing cardiac MYOCD, MEF2C, and ARP5

Myocardin (MYOCD) and MEF2 are essential transcription factors for the heart development and cooperatively regulate the expression of cardiac specific genes through their physical interaction. Actin-related protein 5 (ARP5) is a nuclear localized actin-like protein and involved in the regulation of muscle differentiation. We demonstrate that ARP5 binds to the N-terminus of cardiac isoform of MYOCD protein, which contains MEF2 binding site, and inhibits the interaction between MYOCD and MEF2. To determine the genes regulated by MYOCD-MEF2 and ARP5, we performed RNA sequencing using murine p19 embryonic carcinoma P19CL6 cells exogenously expressing cardiac MYOCD, MEF2C, ARP5, and N-terminal fragment of ARP5.