Conidia were inoculated into 200 ml medium at a concentration of 106 conidia/ml, and incubated at 37°C with shaking (200 rpm) for 36 h. The cell wall was extracted as described under Materials and Met
aRosseti, CA, et. al., manuscript in preparation. bImageJ analysis. (−) gene or protein is down regulated. 16M- Brucella melitensis 16M. N/A- not available.
10-30 ml subsamples were concentrated on 25 mm black polycarbonate filters (0.6 µm for NF, 0.2 µm for B), stained with DAPI for 10 min and filtered. NF were counted using UV and...
