Single-cell RNA-sequencing of virus-specific GP33+CD8+ T cells from day 33 post-LCMV Cl13 infection

CD4+ T cells play a critical role in sustaining the effector function of CD8+ T cells during chronic viral infection. When CD4+ T cell “help” is absent, CD8+ T cells enter a dysfunctional state, losing their capacity for viral control. However, when CD4+ T cell help is present, a heterogenous population of virus-specific CD8+ T cells are present. Here, we applied scRNA-seq to distinguish CD8+ T cell heterogeneity during LCMV Clone 13 infection. GP33+ CD8+ T cells were FACS-sorted from LCMV Cl13-infected mice on day 33 post- infection (2 mice) and were loaded on the Chromium Controller (10x Genomics). Single-cell RNA-seq libraries were loaded on the Chromium Controller (10x Genomics). Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5’ v2 Reagent Kit (10x Genomics) according to manufacturer’s protocol