NNRTI-resistance results obtained through phenotypic testing, direct sequencing and ultra-deep sequencing (UDPS).

ETR = etravirine; Direct = Direct Sanger sequencing; UDPS = Ultra-Deep Pyrosequencing; Stan = Stanford; Tibo = Tibotec; Mono = Monogram. *figure beneath amino acid indicate percentage consisting of a mutated population as determined by UDPS. ** Median (IQR) nucleotide coverage per patient. aFold difference compared to IC50 value of BH10-wild-type (1.0±0.18 µM), which was included in every run. A fold-change of <5 was considered as sensitive, between 5–15 as decreased susceptibility, and above 15 as resistant. A Stanford score of 0-<15, ≥15-<60 and ≥60 are defined as susceptible (s), intermediate (i) and resistant (r). Tibotec weighted genotypic score of 0–2, 2.5–3.5 and ≥4 are predictive of susceptible (s), intermediate (i) and reduced response (r). Monogram defines a weighted score of 0–3 as susceptible (s) and ≥4 as resistant (r).