Transcriptional remodeling by OTX2 directs specification and patterning of mammalian definitive endoderm [scRNA_PS_Meso]

We discovered a role for the transcription factor OTX2 in formation of mouse definitive endoderm (DE). We determined the gene expression profiles during conversion of EpiSCs to Primitive Streak (PS) and Paraxial Mesoderm (PM) using scRNA-seq. We used dTAG13 to deplete OTX2 at PS. Overall design: Mouse EpiSCs were differentiated to Primitive Streak (PS) and Paraxial Mesoderm (PM) with or without dTAG13 added at primitive streak or 24hr DE stage to deplete OTX2 for further 48hrs.