Transepithelial sodium transport in neurosurgical patients with renal salt wasting

This dataset contains the results of experiments measuring the transport of sodium across the monolayers. 22Na was used to make these measurements. The experiments tested the effects of resuspended urinary precipitates from renal salt wasting (RSW) patients and non-RSW Control patients. The amount of protein in the precipitates was used to quantitate the precipitates.
The 'Summary' sheet tabulates the results of the individual experiments described below. The heading numbers such as 5ug or 10ug refer to the concentrations of resuspended precipitates (in micrograms protein/ml) to which the cultures were exposed. In all experiments, cultures were exposed to precipitates on both the mucosal and serosal sides. Numbers tabulated are the effects of various concentrations of precipitate on transepithelial Na movement, expressed as percentages relative to that seen side-by-side in vehicle-only wells.
The control and RSW sheets contain the raw data and calculations, for 47 individual experiments in which the effects of precipitates from the urine of RSW-afflicted patients or non-RSW Control patients on sodium transport across cultured LLC monolayers were measured. Effects of the precipitates on sodium movements were calculated as described in the Methods section of the paper, in brief, the effect of treatment (change in the slope of the linear regression line of sodium movement after treatment versus before) in experimental culture wells versus the effect of vehicle treatment (change in the regression line after versus before treatment) seen in side-by-side Vehicle wells. The results for individual patients are grouped in sub-folders with the patients identified by index numbers.
Other numbers quoted are percent change in transepithelial sodium transport (caused by addition of urinary precipitate, indexed by its protein content) relative to vehicle addition, as described in Methods in body of the paper. (Briefly, relative to Na movement in side-by-side culture wells in which only vehicle was added to the transport medium).

本数据集收录了测量钠离子穿越单层膜运输的实验结果。实验中采用了放射性同位素^22Na进行测量。实验旨在检验来自肾盐丢失（RSW）患者及非RSW对照组患者的尿液沉淀物再悬浮对钠离子运输的影响。沉淀物中的蛋白质含量被用于量化沉淀物。

'总结'标签页以表格形式展示了下述单个实验的结果。标题中的数字，如5ug或10ug，指的是暴露于培养中的沉淀物浓度（以每毫升蛋白质微克数表示）。在所有实验中，培养物均暴露于沉淀物的黏膜和浆膜两侧。表格中的数字表示不同浓度沉淀物对跨上皮钠离子转运的影响，以与仅添加载体的对照孔相比的百分比表示。

对照组和RSW标签页包含了47个单独实验的原始数据和计算结果，这些实验测量了来自RSW患者或非RSW对照组患者尿液中的沉淀物对培养的LLC单层细胞钠离子转运的影响。沉淀物对钠离子运动的影响按照论文中的'方法'部分所述进行计算，简而言之，即实验培养孔中治疗后钠离子转运线性回归线的斜率变化与治疗前相比的变化，以及对照孔中载体治疗后回归线的变化。

个体患者的实验结果被分组在子文件夹中，患者通过索引编号进行标识。文中引用的其他数字是指相对于载体添加引起的跨上皮钠离子转运百分比变化（由尿液沉淀物的蛋白质含量进行索引），具体方法在论文正文中的'方法'部分有详细描述。（简言之，相对于仅添加载体的对照培养孔中的钠离子运动。）