Mouse gut microbiome pre- and post- perturbation (assemblies and MAGs)

Assemblies and MAGs generated from conventional mouse fecal pellets collected pre- and post- gut microbiome perturbation.
, We generated transductomics datasets from mouse fecal samples collected during an experiment that followed a well-established disease model in which 4-5 week old, male, C57BL/6J mice (n=4) were treated with antibiotics prior to challenge with Clostridium difficile. We collected fecal pellets from the mice immediately before (Pre-ABX) and after an antibiotic treatment (Post-ABX) in which cefoperazone (0.5 g/mL) was provided in the drinking water for 5 days. After 2 days of antibiotic-washout with fresh drinking water, the mice were orally gavaged with ~1.0e5 C. difficile (strain 630) at which point we started monitoring the mice for clinical signs of C. difficile infection (CDI). On day 4 of CDI, when toxin-mediated inflammation is known to be severe, we collected a final round of fecal pellets from all mice. We homogenized the pellets from each mouse in each sample condition individually and extract the whole-community DNA with the Qiagen PowerFecal Pro kit.
, # Mouse gut microbiome pre- and post- perturbation (assemblies and MAGs)

Dataset DOI: [10.5061/dryad.76hdr7t9b](https://doi.org/10.5061/dryad.76hdr7t9b)

## Description of the data and file structure

We generated transductomics datasets from mouse fecal samples collected during an experiment in which 4-5 week old, male, C57BL/6J mice (n=4) were treated with antibiotics prior to challenge with *C. difficile*. We collected fecal pellets from the mice immediately before and after an antibiotic treatment in which cefoperazone (0.5 g/mL) was provided in the drinking water for 5 days. After 2 days of antibiotic-washout with fresh drinking water, the mice were orally gavaged with ~1.0e5 C. difficile (strain 630). On day 4 of *C. difficile* infection (CDI), when toxin mediated inflammation is severe, we collected a final round of fecal pellets from all 4 mice. Each sample collection consisted of two fecal pellets from each mouse replicate. The pellets were manually homogenized in 2mL of SM bu..., ,