Impact of 3D-printed perfusable synthetic vasculature on stem-cell based early-stage cerebral organoids

Stem-cell based cerebral organoids were integrated with 3D-printed perfusable synthetic vasculature. The impact of perfusion was evaluated on month-old cerebral organoids was analyzed by comparing the molecular-level changes in perfused and not-perfused vascularized cerebral organoids Overall design: The cerebral organoids were grown on the custom-made synthetic perfusion platform for around a month in two different conditions- with or without continuous media perfusion. For sequencing, the vascularized cerebral organoids were dissociated using the neural tissue dissociation kit (manufacturer: Miltenyi). The plastic debris from the synthetic vasculature were removed using a bead column before sending the single cells for sequencing.