Single cell RNA-seq developmental series in mouse hypothalamus in wild-type and Eed-cKO

Neuronal sub-type diversity in the hypothalamus is staggeringly immense. To investigate the role of epigenetic regulation in hypothalamic development in the generation of this cellular diversity; a floxed Eed allele was crossed to the early, pan-CNS Cre deleter Sox1-Cre, resulting in loss of H3K27me3 in the entire Mus musculus CNS by E11.5. E12.5, E13.5, E15.5, and E16.5 embryonic mouse hypothalamic cells were dissected and analysed by single cell RNA sequencing, on the Illimina/BioRad platform. Control and mutant Eed conditional knock-out at embryonic time points E12.5, E13.5, E15.5, and E16.5 were analysed by scRNA-seq in the mouse hypothalamus.