Next Generation Sequencing Facilitates Quantitative Analysis of healthy donor- and LMNA R527C mutant patient-derived MSCs Transcriptomes

We reprogramed patient derived PBMCs into iPSCs, and differentiated the iPSCs into Mesenchymal stem cells. We performed whole genome wide RNA-seq of patient-derived MSCs and control MSCs. Up-regulated and down-regulated genes of LMNAR527C/R527C patient-derived MSCs were enriched and analyzed. Overall design: To elucidate the mechanism by which Lamin A R527C mutation caused senescence, we performed whole genome wide RNA-seq of patient-derived MSCs and control MSCs, and compared the differentially expressed genes between LMNA R527C mutant and wild type. mRNA profiles of healthy donor- and LMNA R527C mutant patient-derived MSCs