Data from: Draft Genome Assembly of Passalora sequoiae a Needle Blight Pathogen on Leyland Cypress

The objective was to generate a high-quality draft assembly of the whole genome as a resource for future applications such as temporal and spatial dispersal studies of the pathogen and to investigate genotype diversity relevant in fungicide resistance studies and cypress breeding programs. We report here the genome sequence of Passalora sequoiae 9LC2 that was isolated from Leyland cypress 'Leighton Green' (Cupressocyparis leylandii) in 2017 in southern Mississippi. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 x coverage and were used to confirm accuracy of the consensus sequences.

The figures and methods files are documentation in support of a BMC Data Notes publication 'Draft Genome Assembly of Passalora sequoiae a Needle Blight Pathogen on Leyland Cypress'. The images illustrate

Leyland cypress tree showing Passalora twig blight symptoms
Infected Leyland cypress leaf with sporulating conidioma of Passalora sequoiae
Conidia of Passalora sequoiae
Passalora 9LC2 phylogeny

.

Resources in this dataset:
Resource Title: Fig 1 Leyland cypress blight symptoms.
File Name: Fig1 LC blight.JPG
Resource Description: Leyland cypress tree showing Passalora twig blight symptoms.

Resource Title: Fig 2 Passalora sporulation.
File Name: Fig2 LC sporulation.png
Resource Description: Fig. 2. Infected Leyland cypress leaf with sporulating conidioma of Passalora sequoiae.

Resource Title: Fig 4 Passalora 9LC2 phylogeny.
File Name: Fig 4 Passalora 9LC2 phylogeny.pdf
Resource Description: See Methodology for details about generating the phylogenetic tree.

Resource Title: Fig 3 Passalora conidia.
File Name: Fig 3 Passalora conidia.jpg
Resource Description: Fig. 3. Conidia of *Passalora sequoiae*.

Resource Title: Methodology.
File Name: Copes Methodology.docx
Resource Description: This is a more detailed account of methods used in the 'Draft Genome Assembly of *Passalora sequoiae* a Needle Blight Pathogen on Leyland Cypress'.
Resource Software Recommended: Microsoft WORD,url: http://www.microsoft.com