Transcriptomic profilings of bovine mammary epithelial cells stimulated by ten different essential amino acids

In order to investigate the transcriptomic responses of bovine mammary epithelial cells to various essential amino acid administrations Overall design: Bovine mammary epithelial cells cultured in 6-well plates were first serum-starved overnight and subsequently assigned to 1 of 12 treatment media (n = 6). DMEM-F12 medium is the positive control treatment (POS), while DMEM-F12 medium devoid of all essential amino acids served as the negative control treatment (NEG). 10 treatments were NEG individually supplemented with arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan or valine. Individual essential amino acid were supplemented to achieve concentrations equal to those of POS. After 6-h treatment, all cell samples were taken at the same time, frozen in liquid nitrogen, and stored at -80°C until required.