Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation

This includes 1- the data set collected at 100mW = 0.04 kW/cm^2 for the Cy3-Cy5 dye pair that is reported in the paper in Figure 2c and SI Figure 14ab (Supplementary Additional Data 1) 2- Matlab script (zetacorrect.m) to do the simplified zeta correction and a Mathematica notebook (TestingSimplifiedZeta.nb) to show the derivation of simplified zeta correction 3- the raw data corresponding to Figure 2c (Supplementary Additional Data 2) 4- Main Text and Supplementary Figures Source Data ----## Citations If you use any of these shared materials, please use the relevant citatations: ``` * Avik K. Pati^{*}, Zeliha Kilic^{*}, Maxwell I. Martin^{#}, Daniel S. Terry^{#}, Alessandro Borgia, Sukanta Bar, Steffen Jockusch, Roman Kiselev, Roger B. Altman, Scott C. Blanchard, Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation,2024,Nature Methods ``` ----## Relevant Publications In our smFRET data analysis, we used SPARTAN* Single-molecule imaging of non-equilibrium molecular ensembles on the millisecond scale: [paper](https://www.nature.com/articles/nmeth.3769)