Accompanying data for the PhD thesis 'Nanomaterial safety for microbially-colonized hosts'

Name: Accompanying data for the PhD thesis 'Nanomaterial safety for microbially-colonized hosts'
Creator: Brinkmann, Bregje W.
Published: 2022-12-22T00:00:00+08:00

Zenodo2022-12-22 更新2026-04-07 收录

纳米毒理学

斑马鱼模型毒性评估

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https://zenodo.org/record/7066691 数据链接链接失效反馈

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资源简介：

These files include all data presented in chapter 6 of the dissertation: "Nanomaterial safety for microbially-colonized hosts: microbiota-mediated physisorption interactions and particle-specific toxicity" by Bregje Brinkmann (2022). The data presented in chapters 2-5 have previously been published elsewhere: Chapter 2: Zenodo (DOI: 10.5281/zenodo.6800734). Chapter 3: Mendeley Data (DOI: 10.17632/2d4hcr5cb5.1) Chapter 4: Figshare (DOI: 10.6084/m9.figshare.c.4923261) Chapter 5: Mendeley Data (DOI: 10.17632/4nfg69v8hy.1) 1. Data presented in Figure 6.1: Survival_CFU_(...) Tab-delimited file with zebrafish larvae survival, and the number of colony-forming units (CFUs) associated with zebrafish larvae, following exposure to silver nanoparticles (nAg) from 3-5 days-post fertilization (dpf): Concentration: Nominal exposure concentration (mg nAg·L-1). Date: The date at which the mortality was scored (Format: DD/MM/YYYY). Family: A code referring to the aquarium of wildtype zebrafish (ABxTL) that were crossed to obtain the larvae for the experiment. Survival: Percentage of larvae that had survived the treatment. CFU: Number of colony-forming units that was isolated per larva The methodology for toxicity tests and the procedures to determine CFU counts, have been published in Nanotoxicology: Brinkmann BW, Koch BEV, Spaink HP, Peijnenburg WJGM, Vijver MG. 2020. Colonizing microbiota protect zebrafish larvae against silver nanoparticle toxicity. Nanotoxicology. 14: 725-739. DOI: 10.1080/17435390.2020.1755469 2. Data presented in Figure 6.2: ABs_DoseResponses_(...) Tab-delimited file with zebrafish larvae mortality following a pretreatment of 0, 6 or 72 hours with an antibiotic and antifungal cocktail, and subsequent exposure to nAg from 3-5 dpf: Concentration: Nominal exposure concentration (mg nAg·L-1). Mortality: Percentage of larvae that had died. Date: The date at which the mortality was scored (Format: DD/MM/YYYY). Family: A code referring to the aquarium of wildtype zebrafish (ABxTL) that were crossed to obtain the larvae for the experiment. ABs: Duration of the antibiotic/ antifungal pretreatment, either 0, 6 or 72 hours. 3. Data presented in Figure 6.3: il1beta_eGFP_(...) Three folders comprising fluorescence microscopy images (TIFF format) of il1beta:eGFP reporter zebrafish larvae at 5 dpf: (...)_replicates1_20200226: images for the first experimental replicate. (...)_replicates2_20200304: images for the second experimental replicate. (...)_replicates3_20200318: images for the third experimental replicate. For each of the replicates, the following images were acquired: nZnO_GFP: GFP signal for larvae exposed to nZnO. Znion_GFP: GFP signal for larvae exposed to zinc ions. nZnO_trans: transmitted light images for larvae exposed to nZnO. Znion_trans: transmitted light images for larvae exposed to zinc ions. Additionally, the following images have previously been deposited to Mendeley Data (DOI: 10.17632/4nfg69v8hy.1): nAg_GFP: GFP signal for larvae exposed to nAg. nAg_trans: transmitted light images for larvae exposed to nAg. Agion_GFP: GFP signal for larvae exposed to silver ions. Agion_trans: transmitted light images for larvae exposed to silver ions. control_GFP: GFP signal for control larvae that had not been exposed to silver ions or nAg control_trans: transmitted light images for control larvae that had not been exposed to silver ions or nAg. All image processing steps have been published in Ecotoxicology and Environmental Safety: Brinkmann BW, Koch BEV, Peijnenburg WJGM, Vijver MG. 2022. Microbiota-dependent TLR2 signaling reduces silver nanoparticle toxicity to zebrafish larvae. Ecotox Environ Saf. 237: 113522. DOI: 10.1016/j.ecoenv.2022.113522 Abbreviations: ABs: antibiotics CFU: colony-forming units dpf: days post-fertilization il1beta: interleukin-1beta nAg: silver nanoparticles (NM-300 K) nZnO: zinc oxide nanoparticles (NM-110)

提供机构：

Brinkmann, Bregje W.

创建时间：

2022-12-22

Accompanying data for the PhD thesis 'Nanomaterial safety for microbially-colonized hosts'

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