RNA analysis of overexpression of a splicing isoform of PD-1

We overexpressed PD-1^28 in Jurkat cells and performed RNA-sequencing (RNA-seq). Overall design: Targeting the programmed cell death 1 (PD-1) receptor and PD-1 ligand 1 (PD-L1) have achieved some benefits in various cancer types. However, the majorities of cancer patients are less responsive. It is urgent to identify new immune checkpoint (IC) genes and dissect their potential mechanisms. PD-1 receptor maintains peripheral immune tolerance and mediates immune escape of tumor cells as an immune checkpoint. Whether PD-1 splicing variants mediate resistance to PD-1/ PD-L1 blockade remains largely unknown.We identified an alternative splicing isoform of human PDCD1 with a 28-base pair retention from the start of intron 2, encoding PD-1^28.To explore the potential underlying function of PD-1^28 in T cells and to further gain insights into the downstream signaling pathway, we performed RNA-sequencing (RNA-seq) in PDCD1^28 OE cells.