Quantitative proteomic and phosphoproteomic profiling of Sh3rf3-deficient ASD mouse model via 6Plex-TMT and phosphoproteomic

This study aims to systematically investigate the molecular mechanisms underlying autism spectrum disorder (ASD) by analyzing the proteome and phosphoproteome of a Sh3rf3-deficient mouse model. SH3RF3 (SH3 domain-containing RING finger protein 3) is implicated in synaptic regulation pathways, yet its role in ASD remains poorly understood. Through integrated quantitative proteomics and phosphoproteomics, we seek to:Identify dysregulated proteins and phosphorylation events in Sh3rf3 knockout mice.Uncover signaling pathways and post-translational modifications (PTMs) associated with ASD pathophysiology.Provide a resource for understanding synaptic protein networks regulated by SH3RF3.