SMRT- and Illumina-based RNA-seq data of Fenneropenaeus chinensis

We randomly picked two male and two female shrimps of Fenneropenaeus chinensis at 5-months-old. Muscle, gonad, hepatopancreas, intestine, ganglion, heart, and sputum were collected and extracted total RNA using Trizol (Invitrogen, USA). Twenty-eight RNA samples (4 individuals×7 tissues) were mixed into one pool with equal amount of nucleic acids. The mixed pool was used for single-molecule full-length transcriptome sequencing by PacBio platform.Another 15 female and 15 male shrimps were chosen to perform Illumina-based RNA-seq. We collected their gonad and muscle and extracted total RNA using Trizol (Invitrogen, USA). Each 5 RNA samples of same sex and same tissue were mixed into one pool. The 3 pools of same tissue and sex were treated as biological duplicates.