Table_2_Transcriptome Analysis of Long Noncoding RNAs and mRNAs in Granulosa Cells of Jinghai Yellow Chickens Illuminated With Red Light.XLSX

Jinghai Yellow chickens are a new indigenous breed with a dual purpose in China, but their egg laying performance is limited. Compared with white light (WL), exposure to red light (RL) can improve the egg laying performance of hens. Herein, to elucidate the molecular mechanism by which RL affects the egg laying performance, RNA sequencing was used to analyze long noncoding RNAs (lncRNAs) and mRNAs from granulosa cells of small yellow follicles from Jinghai Yellow chickens in RL and WL groups. A total of 12,466 lncRNAs were identified among the assembled transcripts, of which 168 lncRNAs were significantly different between the RL and WL groups (101 downregulated and 67 upregulated). Additionally, 1182 differentially expressed mRNAs were identified (958 downregulated and 224 upregulated). Integrated network analysis demonstrated that numerous differential mRNAs were involved in follicular development through steroid hormone synthesis, oocyte meiosis, and the PI3K-Akt signaling pathway. The impact of lncRNAs on cis and trans target mRNAs indicates that some lncRNAs play important roles in follicular development of small yellow follicles. The results provide a starting point for studies aimed at understanding the molecular mechanisms by which monochromatic light affects follicular development and egg production in hens.

靖海黄鸡为中国新近培育的双用途本土品种，然其产蛋性能尚显不足。相较于白光（WL），红光（RL）照射可提升母鸡的产蛋性能。本研究旨在阐明RL影响产蛋性能的分子机制，通过RNA测序技术对靖海黄鸡小黄卵泡颗粒细胞的lncRNA和mRNA在RL及WL组别中的表达情况进行分析。在组装的转录本中，共鉴定出12,466个lncRNA，其中RL与WL组别间差异显著的lncRNA有168个（下调101个，上调67个）。此外，还鉴定出1,182个差异表达mRNA（下调958个，上调224个）。综合网络分析显示，众多差异表达的mRNA通过类固醇激素合成、卵母细胞减数分裂以及PI3K-Akt信号通路参与卵泡发育。lncRNA对顺式和反式靶mRNA的影响表明，部分lncRNA在小黄卵泡的发育过程中扮演着至关重要的角色。研究结果为探究单色光影响母鸡卵泡发育及产蛋的分子机制提供了新的研究起点。