DataSheet_2_A Multiparametric Method Based on Clinical and CT-Based Radiomics to Predict the Expression of p53 and VEGF in Patients With Spinal Giant Cell Tumor of Bone.pdf

PurposeThis project aimed to assess the significance of vascular endothelial growth factor (VEGF) and p53 for predicting progression-free survival (PFS) in patients with spinal giant cell tumor of bone (GCTB) and to construct models for predicting these two biomarkers based on clinical and computer tomography (CT) radiomics to identify high-risk patients for improving treatment.Material and MethodsA retrospective study was performed from April 2009 to January 2019. A total of 80 patients with spinal GCTB who underwent surgery in our institution were identified. VEGF and p53 expression and clinical and general imaging information were collected. Multivariate Cox regression models were used to verify the prognostic factors. The radiomics features were extracted from the regions of interest (ROIs) in preoperative CT, and then important features were selected by the SVM to build classification models, evaluated by 10-fold crossvalidation. The clinical variables were processed using the same method to build a conventional model for comparison.ResultsThe immunohistochemistry of 80 patients was obtained: 49 with high-VEGF and 31 with low-VEGF, 68 with wild-type p53, and 12 with mutant p53. p53 and VEGF were independent prognostic factors affecting PFS found in multivariate Cox regression analysis. For VEGF, the Spinal Instability Neoplastic Score (SINS) was greater in the high than low groups, p

本课题旨在评估血管内皮生长因子（VEGF）和p53在预测骨脊性巨细胞瘤（GCTB）患者无病生存期（PFS）中的重要性，并基于临床及计算机断层扫描（CT）影像组学构建预测这两项生物标志物的模型，以识别高风险患者，从而改善治疗方案。研究方法：自2009年4月至2019年1月，对在我机构接受手术的80例骨脊性GCTB患者进行了回顾性研究。收集了VEGF和p53的表达以及临床和一般影像信息。采用多因素Cox回归模型验证预后因素。从术前CT的感兴趣区域（ROIs）中提取影像组学特征，然后通过支持向量机（SVM）选择重要特征以构建分类模型，并通过十折交叉验证进行评估。采用相同的方法处理临床变量，以构建传统模型进行比较。研究结果：80例患者的免疫组化结果如下：VEGF高表达者49例，低表达者31例；野生型p53表达者68例，突变型p53表达者12例。在多因素Cox回归分析中发现，p53和VEGF是影响PFS的独立预后因素。对于VEGF，高表达组的脊柱不稳肿瘤评分（SINS）高于低表达组，p