Chromatin context-dependent effects of epigenetic drugs on CRISPR-Cas9 editing

We sought to find if epigenetic drugs had chromatin context-dependent effects on CRISPR-Cas9 efficiency and DNA double-strand break repair. We performed a multiplexed drug screen in a reporter cell line containing 19 sequencing based pathway reporters. This submission contains high throughput sequencing data of the pathway reporters, their mapping and mutation readout. It also contains the raw pA-DamID data for H3K27me3, H3K9me2, H3K9me3 and "Dam-only" samples in RPE-1 cells. The samples are pA-DamID experiments, as described in (van Schaik , 2022), where DNA-protein interactions are measured. Here we profile H3K27me3, H3K9me2, H3K9me3 and Dam only in hTERT RPE-1 cells. The Dam only samples are used as controls for DNA accessibility. These data were used in this study to compare DNA repair outcomes of DNA double strand breaks in different chromatin states in RPE-1. ChIP-seq experiments have been performed using H3K27ac and H3K27me3 antibodies. 3 biological replicates are provided.