Expression data from E16 mouse embryonic organoids - grown with epithelium and stroma in the presence or absence of FGF2 [Epi]

FGF2 signals BMP7 expression and TGFB/myofibroblast gene inhibition that promotes proacinar differentiation. We have characterized these stromal factors affecting the epithelium using a combination of scRNA Sequencing of whole tissue and transcriptional profiling of the organoid cell compartments. Gene expression changes and GSEA was performed using gene expression microarrays. Organoids made from embryonic mouse cells were grown then different cell types were separated and their transcriptomes were analyzed based on their response to growth conditions with or without FGF2.