BRIN-BD11 cells viability following 1 h incubation in stimuli-supplemented KRBB.

BRIN-BD11 cells were incubated for 1 h in KRBB supplemented with either various concentrations of D-glucose (0–30 mmol/l) in the absence or presence of 10 mmol/l L-alanine or L-alanine () with/without supplementation with 16.7 mmol/l D-glucose. Results are expressed as percentage of control (11.1 mmol/l D-glucose, same concentration of RPMI-1640 media used to maintain cells in culture). Data are mean ± SD (n = 3). * stimuli-free KRBB vs 16.7 mmol/l D-glucose KRBB, + stimuli-free KRBB vs 10 mmol/l L-alanine.