Multi-serotype pneumococcal nasopharyngeal carriage prevalence in vaccine naïve Nepalese children, assessed using molecular serotyping.

Invasive pneumococcal disease is one of the major causes of death in young children in resource poor countries. Nasopharyngeal carriage studies provide insight into the local prevalence of circulating pneumococcal serotypes. There are very few data on the concurrent carriage of multiple pneumococcal serotypes. This study aimed to identify the prevalence and serotype distribution of pneumococci carried in the nasopharynx of young healthy Nepalese children prior to the introduction of a pneumococcal conjugate vaccine using a microarray-based molecular serotyping method capable of detecting multi-serotype carriage. We conducted a cross-sectional study of healthy children aged 6 weeks to 24 months from the Kathmandu Valley, Nepal between May and October 2012. Nasopharyngeal swabs were frozen and subsequently plated on selective culture media. DNA extracts of plate sweeps of pneumococcal colonies from these cultures were analysed using a molecular serotyping microarray capable of detecting r...侵袭性肺炎球菌疾病（Invasive pneumococcal disease）是资源匮乏国家幼儿死亡的主要病因之一。鼻咽定植研究可用于解析当地循环流行的肺炎球菌血清型的流行特征，但目前针对多重肺炎球菌血清型同时定植的相关数据仍极为稀缺。本研究旨在借助可检测多重血清型定植的基于微阵列（microarray）的分子血清分型方法，明确尼泊尔在引入肺炎球菌结合疫苗（pneumococcal conjugate vaccine）前，健康尼泊尔幼儿鼻咽部携带肺炎球菌的流行率及血清型分布情况。我们于2012年5月至10月间，在尼泊尔加德满都谷地开展了一项针对6周至24月龄健康儿童的横断面研究。将采集的鼻咽拭子进行冷冻保存，随后接种于选择性培养基进行培养。对上述培养体系中肺炎球菌菌落的平板洗脱物的DNA提取物，采用可检测……的分子血清分型微阵列开展分析。