Waltraud G. Muller, J. Bernard Haymann, Kunio Nagashima, Peter Guttman, Stephan Werner, Stefan Rehbein, Gerd Schneider, James G. McNally, Stefan Heim, Florian Mueller (2012) CIL:41362, Mus musculus, adenocarcinoma. CIL. Dataset

The image shows a slice through a 3D tomographic reconstruction of a mouse adenocarcinoma cell based on x-ray microscopy. Live cells were cryofixed by plunge freezing in liquid ethane and the vitrified material observed unstained using x-ray microscopy at a wavelength that yields high contrast between water and organic material. Images were taken over a range of specimen tilts from -60 to +60 degrees at 1 degree intervals. The image represents a 9.8 nm thick slice of the computed tomogram in the x-y plane, and shows a region of cytoplasm containing vesicles. The image has a resolution of ~36 nm (Rayleigh Criterion) and ~70 nm (Fourier Ring Correlation), the latter corresponding to ~35 nm half-pitch resolution. Additional X-ray images from the study are included in this CIL Group.
See also: W G Muller et al. 2012 Towards an atlas of mammalian cell ultrastructure by soft X-ray tomography. J Struct Biol 177:179-192.

该图像展示了基于X射线显微镜对小鼠腺癌细胞的3D断层重建切片。活细胞经液态乙烷急速冷冻固定，未经染色，通过X射线显微镜在产生水与有机材料高对比度的波长下进行观察。图像是在-60至+60度的范围内，以1度为间隔的多个标本倾斜角度下拍摄的。图像代表x-y平面上的计算断层扫描的9.8纳米厚切片，并展示了一个包含泡状结构的细胞质区域。该图像的分辨率为约36纳米（瑞利判据）和约70纳米（傅里叶环相关），后者相当于约35纳米的半间距分辨率。本研究中的其他X射线图像已包含在本CIL组中。参见：W G Muller等，2012年，《通过软X射线断层扫描构建哺乳动物细胞超结构图谱》，结构生物学杂志，177卷，179-192页。