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Sensitivity and linear dynamic range of miRNA detection
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2010-06-20
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Primer and probe sequences.
All oligonucleotide sequences are listed in 5′–3′ orientation. M = Matrix, HA = Hemagglutinin, NA = Neuraminidase, n.a. = not applicable.
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Multiplex qPCR Data.
Results of multiplexing trials of qPCR markers. Average Ct values, with the range of copy numbers in parentheses, found across 24 replicates for markers run singly or combined in a multiplex reaction.
Figshare2015-12-03 更新20
Rapid and Sensitive Detection of Bartonella bacilliformis in Experimentally Infected Sand Flies by Loop-Mediated Isothermal Amplification (LAMP) of the Pap31 Gene
BackgroundCarrion' disease, caused by Bartonella bacilliformis, remains truly neglected due to its focal geographical nature. A wide spectrum of clinical manifestations, including asymptomatic bactere
Figshare2016-01-15 更新00
Expression analysis of microRNA by expression microarrays of rectal cancer derived from the Cancer Institute Hospital of Japanese Foundation for Cancer Research.
We performed the expression microarray experiments for miRNA of rectal cancer tissue. microRNA were extracted from primary tumor of 137 rectal cancer patients, hybridized and scanned with Affymetrix m
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Effect of ambient temperature on amplification efficiency and reproducibility in NINA heaters.
*Calculated by accounting for the volume of each linearity panel member added to the reaction tube. †Number of positive samples/total number tested.
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