We optimized a protocol to enrich, digest and add poly(A) tail to the circular RNAs in order to make them compatible with the Oxfor Nanopore Technology for full-length sequencing Circular RNAs from hu
BACKGROUND: Sequencing full-length cDNA clones is important to determine gene structures including alternative splice forms, and provides valuable resources for experimental analyses to reveal the
Pooled mRNA of ten rice cultivars from the subspecies aus, indica and japonica were sequenced on the PacBio Sequel I platform and collapsed with the tool cogent.