Read length simulations with sequencing errors

Sequences of uniform length at each of 100, 150, 200, 250, 300, 350, 400, 500, 600, 700, 800, 1000 and 1200 bp were drawn at random from Populus trichocarpa chromosomes 1–19 (v3.0, DOE-JGI, http://www.phytozome.net/poplar) using 0.1x coverage. Simulations were conducted using PBSIM v1.0.3 with built-in PacBio CCS error profiles (substitutions/insertions/deletions ratio of 6:21:73; read accuracy of 98 ± 2%).