Expression profile of microRNAs in different stages of wound healing in mice

Wound healing response is characterized by progression from clot formation to an inflammatory phase, a repair phase, and finally remodeling. MicroRNAs (miRNAs) are 20-24 nucleotide noncoding RNAs that can affect protein expression at the posttranscriptional level by modulating the stability and translation of corresponding messenger RNAs (mRNAs). The roles that miRNAs play in various physiological and pathological processes have received increasingly widespread attention. However, the differentially expressed miRNAs involved in different stages of wound healing and their roles remain to be further explored. In this study, we established wound model on the shaved dorsum using a punch biopsy tool in C57BL/6J mice. Biopsies were harvested on postoperative days 0, 1, 3, and 7. Then, we identified the differentially expressed miRNAs in different stages of wound healing through Illumina sequencing and miRDeep2 analysis. This study help to find out the expression profile of microRNAs during no...伤口愈合反应以凝血块形成为起始，依次历经炎症阶段、修复阶段，最终进入重塑阶段的进程为典型特征。微小RNA（microRNAs，miRNAs）是一类长度为20-24个核苷酸的非编码RNA，可通过调控对应信使RNA（messenger RNAs，mRNAs）的稳定性与翻译过程，在转录后水平影响蛋白质的表达。miRNAs在各类生理与病理过程中发挥的功能已受到学界越来越广泛的关注。然而，参与伤口愈合不同阶段的差异表达miRNAs及其具体作用机制仍有待进一步探究。本研究使用打孔活检工具，在剃毛后的C57BL/6J小鼠背部构建伤口模型，并分别于术后第0、1、3、7天采集活检组织。随后，通过Illumina测序与miRDeep2分析，我们鉴定出了伤口愈合不同阶段的差异表达miRNAs。本研究有助于阐明microRNAs在未完整表述的过程中的表达谱。