RNA-sequencing of GFP+ cells 2 days post-transduction of LSK cells with Scr or miR-99 KD lentiviral vectors. Mus musculus

We FACS purified GFP+ cells 2 days post-transduction of LSK (Lin-Negative c-Kit+ Sca1+) cells with miR-99 KD and Scr lentiviral vectors and performed RNA-sequencing; this allowed us to identify potential miR-99 target genes for inclusion in the shRNA library Overall design: FACS purification of Scr and miR-99 KD GFP+ cells 2 days after infection, with two to three replicates per experimental group