Protein kinase FaSnRK2.6 phosphorylates transcription factor FabHLH3 to regulate anthocyanin homeostasis during strawberry fruit ripening

Strawberry (Fragaria×ananassa) is a model plant for studying non-climacteric fruit ripening regulated by abscisic acid (ABA). However, the signaling of ABA in the regulation of fruit coloration is not fully understood. Here, a transcription factor FabHLH3 key to fruit coloration is identified by yeast two hybrid library screening using FaSnRK2.6 as a bait, an ABA core signaling component negative to ripening. Indeed, this interaction is also confirmed by firefly luciferase complementation assay and pull-down assay. RT-qPCR and Western blotting analysis confirm FabHLH3 is expressed ubiquitously in strawberry and stably during fruit development. Manipulating both FabHLH3 and FaSnRK2.6 expression by overexpression and interference demonstrates that FabHLH3 and FaSnRK2.6 promote and inhibit strawberry fruit coloration, respectively, using the marker gene FaUFGT, key to anthocyanin biosynthesis. FaSnRK2.6 can phosphorylate FabHLH3, which promotes FaUFGT expression by the directly binding to ..., , , # Protein kinase FaSnRK2.6 phosphorylates transcription factor FabHLH3 to regulate anthocyanin homeostasis during strawberry fruit ripening

[https://doi.org/10.5061/dryad.h70rxwdt5](https://doi.org/10.5061/dryad.h70rxwdt5)

## Description of the data and file structure

It (Sequence.docx) contains the  FabHLH3 fasta sequence、FabHLH3 protein sequence、FaSnRK2.6 fasta sequence、FaSnRK2.6 protein sequence.

草莓（Fragaria × ananassa）是研究脱落酸（abscisic acid, ABA）调控的非呼吸跃变型果实成熟的模式植物。然而，ABA在果实着色调控中的信号机制尚未完全阐明。本研究以脱落酸核心信号通路中负调控果实成熟的组分FaSnRK2.6为诱饵，通过酵母双杂交文库筛选，鉴定得到一个关键的果实着色相关转录因子FabHLH3。随后，通过萤火虫荧光素酶互补实验与pull-down实验验证了二者的相互作用。实时定量聚合酶链式反应（RT-qPCR）与蛋白质免疫印迹（Western blotting）分析证实，FabHLH3在草莓各组织中广泛表达，且在果实发育进程中表达稳定。通过过表达与基因干扰手段同时调控FabHLH3与FaSnRK2.6的表达，并以花青素生物合成关键标记基因FaUFGT为检测指标，证实FabHLH3与FaSnRK2.6分别促进和抑制草莓果实着色。FaSnRK2.6可磷酸化FabHLH3，后者通过直接结合……进而促进FaUFGT的表达。# 研究主题：蛋白激酶FaSnRK2.6磷酸化转录因子FabHLH3调控草莓果实成熟过程中的花青素稳态
数据集DOI：https://doi.org/10.5061/dryad.h70rxwdt5

## 数据与文件结构说明
本次提供的Sequence.docx文件包含FabHLH3的FASTA格式核酸序列、FabHLH3蛋白序列、FaSnRK2.6的FASTA格式核酸序列以及FaSnRK2.6蛋白序列。