Single-cell RNAseq analysis of the empty and i8TF cell lines after 3 days of BL-CFC culture. Single-cell RNAseq analysis of the empty and i8TF cell lines after 3 days of BL-CFC culture

The aim of the experiment was to assess the cell heterogeneity after the doxycycline treatment and the subsequent induction of the 8 transcription factors in the BL-CFC culture. Overall design: A2lox.empty and A2lox.i8TFs cell lines were used for this experiment. A2lox.empty is used a negative control while the doxycycline is inducing the expression of Erg, Fli1, Tal1, Lyl1, Lmo2, Runx1, Cbfb and Gata2 in the A2lox.i8TFs cell line. Cells were cultured in BL-CFC culture for 1 day before doxycycline treatment and were cultured for an additional 48hours with (i8TFs_plus and E_plus) or without doxycycline (i8TFs_minus and E_minus). As additional controls of the single cell RNA seq experiment, bulk A2lox.i8TFs cells were collected at the same time point from the condition without doxycycline (Pos_Ctrl), also buffer only was used as negative control (Neg_Ctrl) and a human cell line as other control (K562).