Gene expression changes in white adipose tissue from Ing1- and Gadd45a- single- or double-knockout mice. Mus musculus

ING1b and GADD45a are nuclear proteins involved in the regulation of cell growth, apoptosis and DNA repair. We previously found that ING1b is essential to target GADD45a-mediated active DNA-demethylation via TET1 to specific loci. Hence, GADD45a and ING1 play a crucial role in epigenetic gene regulation. In order to study the physiological role of ING1-GADD45a on gene expression regulation, we compared the expression profiles of white adipose tissue (WAT) from wildtype, Ing1-/-, Gadd45-/- and double-knockout (DKO) mice. Overall design: WAT gene expression profiling in all 4 genotypes with triplicates consisting of pooled RNA from 3 male mice each.