Transcriptional regulation of the glucose-6-phosphate transporter 2

Arabidopsis Col-0 and WS ecotypes were subjected to a high light treatment and leaf tissues were harvested 0, 15, 30, 60, 120, or 240 after exposure to high light. RNA was isolated and sequenced in order to determine gene expression changes after an increase in light. The transcript for GPT2 transiently increases several hundred-fold within two hours, and the increase in GPT2 transcript is preceded by an increase in expression of many transcription factor genes including Redox Responsive Transcription Factor 1 (RRTF1). It is hypothesized that transient GPT2 expression is adaptive, allowing G6P to move into the chloroplast from the cytosol. The imported G6P can be used for starch synthesis or may flow directly into the Calvin-Benson cycle via an alternative pathway (the G6P shunt), SRP200873; PRJNA548115; Two ecotypes sampled, six time points after start of high light treatment, three biological replicates per sample