Adipose tissue explant culture using PDMS flow chambers: an alternative to static explant culture

As obesity continues to rise, it is important we can effectively study adipose tissue. However, adipose tissue is notoriously difficult to culture ex vivo due to fragility, bouyancy, and high nutrient demant. To address these challenges, we developed a novel approach using polydimethylsiloxane (PDMS) flow chambers attached to a micro peristaltic pump for culture of primary adipose tissue explants. Our results demonstrate that adipose tissue remains viable and functional in flow chambers, therefore providing an alternative to static explant culture in a dish.