Richard Allen (University of Hawaii), C. Schroeder (2011) CIL:13130, Paramecium multimicronucleatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

High resolution image of quick-freeze deep-etch view of a radial arm showing a collecting canal surrounded with smooth spongiome as well as a few decorated tubules. However several larger vesicles lie next to the radial arm containing a lumen resembling the contractile vacuole. These vesicles appear to be rounded decorated tubules which have been transformed into vesicles or have not yet tubulated and become part of the decorated spongiome of the mature radial canal. Although we cannot be certain without further study we believe this may represent a dividing cell as we do know that the radial arms of the dividing cell, both their smooth and decorated spongiomes, shorten as the new CVCs develop (Allen et al., J. Cell Sci. 96:469-475, 1990; Fok et al., J. Eukaryot. Microbiol. 49:185-196, 2002. The P-face of these vesicles has a large number of IMPs like that seen in collecting canals. The highly porous E-face of several vesicles indicates that these vesicles are indeed products of the decorated spongiome. TEM taken on 6/22/88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 9,780X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

高分辨率图像呈现了快速冷冻深蚀切片的辐射臂视图，其中展示了被平滑海绵质围绕的收集管以及数个装饰性细管。然而，紧邻辐射臂的几个较大泡状结构中包含着类似收缩空泡的腔室。这些泡状结构似为圆形装饰性细管，它们可能已转化为泡状结构，或尚未管化，并成为成熟辐射管装饰海绵质的一部分。尽管未经进一步研究我们无法确定，但我们认为这或许代表了一个分裂细胞，因为我们已知分裂细胞的辐射臂，包括其平滑和装饰性海绵质，在新的收缩空泡形成过程中会缩短（参见Allen等人的《细胞科学杂志》第96卷第469-475页，1990年；Fok等人的《真核微生物学杂志》第49卷第185-196页，2002年）。这些泡状结构的P面具有大量类似收集管中观察到的内质颗粒（IMP）。几个泡状结构的致密E面表明，这些泡状结构确实为装饰性海绵质的产物。该透射电子显微镜（TEM）图像由C. Schroeder于1988年6月22日使用80kV的Zeiss 10A设备拍摄，负片放大倍数为9,780倍。原始胶片使用Epson Perfection V750 Pro扫描仪进行扫描。此图像最适用于定量分析。更多详细信息请访问（http://www5.pbrc.hawaii.edu/allen/）。