TOXINS GENES AND METHODS OF USING THEM

FIELD: biotechnology. SUBSTANCE: invention discloses a recombinant nucleic acid molecule to provide higher insecticidal resistance or tolerance of Lepidopteran insect pests. Said nucleic acid molecule codes the amino acid sequence of Bacillus thuringiensis toxin. Also disclosed is a recombinant polypeptide with insecticidal activity， encoded by said nucleic acid molecule， including in composition for providing higher resistance or tolerance to Lepidopteran insect pests. Nucleic acid molecule of the invention is used in expression cassettes for transformation and expression in bacterial and plant host cells. Invention also relates to transgenic plants and seeds containing a nucleic acid molecule according to the invention， to a method of protecting plants against Lepidoptera insect pests and a method for increasing plant yield. EFFECT: invention allows to improve crop capacity by insect pests control. 20 cl， 3 tbl， 9 ex