Transcription profiling of the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates

The aim of this experiment was to use global gene expression profiling to identify genes that are differentially expressed in the islets from Pdx1+/- mice compared to islets isolated from Pdx1 +/+ littermates. The Pdx1 null mutation consists of a nuclear targeted _-galactosidase cassette fused in-frame with the N terminus of PDX-1.本实验旨在通过全基因组基因表达谱分析，鉴定胰十二指肠同源框基因1（Pdx1）杂合突变（Pdx1+/-）小鼠胰岛，相较于同窝野生型（Pdx1+/+）小鼠分离得到的胰岛中差异表达的基因。Pdx1无效突变指一段核靶向的_半乳糖苷酶（_-galactosidase）表达盒与PDX-1的N端进行框内融合。