Convergence of BMI1 and CHD7 on ERK signalling in medulloblastoma

We describe a novel molecular convergence between BMI1 and CHD7 in the initiation of medulloblastoma. We show that CHD7 controls differentiation of G4 MB cells and its silencing renders them susceptible to Bmi1-mediated ERK1/2-induced proliferation control. We show that BMI1-mediated repression of the ERK1/2 inhibitor DUSP4 leads to increased proliferation and tumour burden in primary human MB cells. Overall design: Two biological replicates of the following knockdown conditions were used: shBMI1, shCHD7, double shBMI1/shCHD7, and scrambled controls.