Supplemetary material final draft.docx

Evaluation of intestinal response to zinc restriction in pigs.Supplementary table 1 shows the growth performance of pigs in different treatment groups. Supplementary tables 2 and 3 show the differentially expressed genes (DEGs) in ZnR compared with ZnCl100 and ZnCl50 respectively.Supplementary tables 4-11 show the panther analysis of the DEGs. Supplementary figure 1 shows top three upregulated and downregulated canonical pathways through IPA analysis of differentially expressed genes in ZnCl100 compared with ZnCl50. Z-score >2 or <-2 were considered significantly upregulated (black columns) or downregulated (grey columns) respectively. n=8 for ZnCl100 and ZnCl50. Supplementary figure 2 shows independent examination of tissue samples by qPCR revealed significant changes similar to log2 fold change values in DEGs. * Mann-Whitney unpaired non-parametric test (p<0.05 was considered significant). n=7 for ZnR, n=8 for ZnCl100 and ZnCl50.