Nanostring gene expresson-based, antibody activation of bone marrow derived macrophages (BMDM)

We study immune responses induced by amyloid targeting antibodies and CAA-mediated microhemorrhages using histology and gene expression analyses in AD mouse models and primary culture settings. We cultured and plated primary bone marrow-derived macrophages in wells coated with high Fc receptor activating antibody IgG2a or isotype control antibody harboring the LALAPG mutation, ablating Fc-FcR mediated effector functions To compare the transcriptional profiles for BMDM activated by Fc receptor signaling, isotype control antibody or non-treated.