five

Characterization of the RNA content of chromatin

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NIAID Data Ecosystem2026-03-10 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP002245
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We deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harbouring CARS. Overall design: We purified CARs from normal HFs by isolating soluble chromatin after MNase treatment, followed by separation of chromatin fragments of different lengths on a sucrose gradient. CARs were converted into double-stranded cDNAs and sequenced using the Illumina Genome Analyzer I.
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2017-09-17
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