Genome-wide analysis of epidermal gene expression from Presenilin (1&2)-null, RBPjk-null and wild-type litter mates

Analysis of differential gene expression in Psen-null and RBPjk-null epidermal keratinocytes. The hypothesis tested in the present study was that canonical Notch signals could be present at the beginning of epidermal stratification (E13.5) in the RBPj-null epidermis. If an early signal was preserved by epigenetic memory, E18.5 RBPj-null epidermis would resemble the wild-type at the transcriptional level. Total RNA obtained from 3xRBPj-null and 3xPsen-null epidermis at E18.5 and compared to wild-type (3xRBPjwt & 3xPsenwt) epidermis from littermates.本研究针对Psen基因敲除（Psen-null）与RBPjk基因敲除（RBPjk-null）表皮角质形成细胞中的差异基因表达展开分析。本研究验证的核心假说为：在表皮分层起始阶段（胚胎发育第13.5天，E13.5），经典Notch信号通路可在RBPj基因敲除表皮中存在。若早期信号可通过表观遗传记忆得以保留，则胚胎发育第18.5天（E18.5）的RBPj基因敲除表皮在转录水平上将与野生型表皮高度相似。本研究从胚胎发育第18.5天的3组RBPj基因敲除表皮与3组Psen基因敲除表皮中提取总RNA，并将其与同窝野生型（3xRBPjwt与3xPsenwt）表皮进行对照比较。