The Role of Uridine Glucuronosyl transferase 2B28 (UGT2B28) in Prostate Cancer Progression [day30]

To understand the effect of UGT2B28 on tumor growth, we generated lentiviral-mediated UGT2B28 knockdown and rescue (by overexpressing UGT2B28 in the knockdown background) in LAPC4 cells. These engineered cells were then subcutaneously injected in athymic nude mice with pre-implanted testosterone pellets. We then performed transcriptomics on the xenograft tumors upon resection at ~30 days Overall design: Comparative gene expression profiling analysis of RNA-seq data for LAPC4 control (NT), LAPC4 KD (2B28 knockdown), and LAPC4 rescue (overexpression of 2B28 in KD background)