RNA-seq of iNKT cells in the skin and spleen of CCR10+/EGFP mice

To compare transcriptomic profiles between skin and spleen iNKT cells, the analysis of global gene expressions in iNKT cells was performed using RNA-seq data obtained from iNKT cells purified from skin and spleen of CCR10+/EGFP mice. Total RNA of iNKT cells were isolated. RNA-sequencing was done for the transcriptomic analysis of skin and spleen iNKT cells.为比较皮肤与脾脏来源的不变自然杀伤T细胞（iNKT cells）的转录组谱，本研究采用从CCR10+/EGFP小鼠皮肤和脾脏中纯化的iNKT细胞所获得的RNA测序（RNA-seq）数据，开展iNKT细胞的全局基因表达分析。研究人员分离了iNKT细胞的总RNA，并通过RNA测序完成皮肤与脾脏iNKT细胞的转录组分析。