Effect of decitabine on gene expression in THP-1 or decitabine-resistant THP-1 leukemia cells

To globally evaluate transcriptome in THP-1 and decitabine-resistant THP-1 cells treated by decitabine, cells were treated with the IC90 dose of decitabine until 90% cell death was reached, then cultured in decitabine-free medium until confluence, followed by another round of decitabine treatment and the treatment cycles were repeated for 3 months to generate decitabine-resistant THP-1 cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of THP-1 or decitabine-resistant THP-1 cells treated with or without decitabine. Comparative gene expression profiling analysis of RNA-seq data for THP-1 or decitabine-resistant THP-1 cells treated with or without 1μM decitabine for 6 days.